Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells

Melon (Cucumis melo L.) has high economic value and in recent years, its production has increased; however, part of the fruit is wasted. Usually, inedible parts such as peel and seeds are discarded during processing and consumption. Extracts of melon residues were prepared and their phenolic compounds, antioxidants and antiproliferative activities were evaluated. Total phenolic compounds were found in hydroethanolic, hydromethanolic, and aqueous extracts, especially for melon peel (1.016 mg gallic acid equivalent/100 g). Flavonoids total content found for melon peel aqueous extract was 262 µg of catechin equivalent (CA)/100 g. In all extracts of melon peel significant amounts of gallic acid, catechin, and eugenol were found. For total antioxidant capacity, reported as ascorbic acid equivalent, the hydroethanolic and hydromethanolic extracts in peels and hydromethanolic in seeds were 89, 74, and 83 mg/g, respectively. Different extracts of melon showed iron and copper ions chelating activity at different concentrations, especially melon peel aqueous extract, reaching values of 61% for iron and 84% for copper. The hydroethanolic extract of melon peel presented a significant ability for hydroxyl radicals scavenging (68%). To assess the antiproliferative potential in human cancer cell lines, such as kidney carcinoma, colorectal carcinoma, cervical adenocarcinoma and cervical carcinoma, MTT assay was performed. The proliferation was inhibited by 20-85% at extracts concentrations of 0.1-1.0 mg/mL in all cancer cell lines. The results suggest that melon residues extracts display a high antioxidant activity in in vitro assays and have effective biological activity against the growth of human tumor cells.

Research progress in heavy metal toxic damage of nervous system / 中国药理学与毒理学杂志

With the development of modern industry,heavy metal pollution in China is becoming increasingly serious.Large-scale water sources and farmland have been severely contaminated.Frequent contact with heavy metal in life or at work has caused a marked increase in heavy metal poisoning in recent years.Nervous system damage caused by heavy metal poisoning is extremely common in clinic,and poses a threat to a patient's life,bringing heavy burden to society and families.Therefore,early prevention and treatment are crucial.This article takes commonpoisoning several common heavy metals as an example and combine the domestic and foreign research to review the damage to the nervous system caused by heavy metal poisoning.We aim to make patients and medical staff more alert to such poisoning and provide the basis for clinical prevention,diagnosis,treatment and research.

Improved synthesis of dipyridyl thioselenosemicarbazones / 国际药学研究杂志

Objective To improve the synthesis process of N-allyl-2-(di(pyridin-2-yl)methylene)hydrazinecarboseleno?amide(4)and 2-(di(pyridin-2-yl)methylene)-N,N-dimethylhydrazinecarboselenoamide(8). Methods 4-allyl-3-thiosemicarbazide (1)was the starting material,which reacted with methyl iodide,sodium hydrogen selenide to obtain intermediate 4-allyl-3-selenosemi?carbazide(3). Compound(3)reacted with di-2-pyridyl ketone to obtain the target compound 4. 4,4-Dimethyl-3-thiosemicarbazide was the starting materials,which reacted with di-2-pyridyl ketone to obtain intermediate dipyridyl thiosemicarbazone(6). Compound 6 re?acted with methyl iodide,sodium hydrogen selenide to obtain the target compound 8. We optimized the reaction conditions of each step. Results We synthesized the target compound 4 and 8 according to the respective synthetic route,the structure of the target com?pounds and the intermediates were confirmed by 1H NMR and MS. Conclusion The improved processes are easy to operate,cost-saving and suitable for large scale preparation.

Improved synthesis of dipyridyl thioselenosemicarbazones / 国际药学研究杂志

Objective To improve the synthesis process of N- allyl- 2-(di(pyridin- 2- yl)methylene)hydrazinecarboselenoamide(4)and2-(di(pyridin-2-yl)methylene)-N,N-dimethylhydrazinecarboselenoamide(8). Methods 4-allyl-3-thiosemicarbazide (1)was the starting material,which reacted with methyl iodide,sodium hydrogen selenide to obtain intermediate 4-allyl-3-selenosemicarbazide(3). Compound(3)reacted with di-2-pyridyl ketone to obtain the target compound 4. 4,4-Dimethyl-3-thiosemicarbazide was the starting materials,which reacted with di-2-pyridyl ketone to obtain intermediate dipyridyl thiosemicarbazone(6). Compound 6 reacted with methyl iodide,sodium hydrogen selenide to obtain the target compound 8. We optimized the reaction conditions of each step. Results We synthesized the target compound 4 and 8 according to the respective synthetic route,the structure of the target compounds and the intermediates were confirmed by 1H NMR and MS. Conclusion The improved processes are easy to operate,cost-saving and suitable for large scale preparation.